Disentangling the diet composition of Arctic shorebirds’ chicks provides a new perspective on trophic mismatches Mikhail K. Zhemchuzhnikov1, Elena A. Zhemchuzhnikova1,2, Thomas K. Lameris1, Judith van Bleijswijk2, Viktor Golovnyuk3, Job ten Horn1, Dmitry Kutcherov4, Anastasiya Popovkina3, Mikhail Soloviev3, Maria Sukhova3,5, Harry J. Witte2 and Jan A. van Gils1,6* 1 Department of Coastal Systems, NIOZ Royal Netherlands Institute for Sea Research, PO Box 59, 1790 AB Den Burg, The Netherlands 2 Department of Marine Microbiology & Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, PO Box 59, 1790 AB Den Burg, The Netherlands 3 Department of Vertebrate Zoology, Lomonosov Moscow State University, Moscow, 119991, Russia 4 Department of Entomology, St. Petersburg State University, St. Petersburg, 199034, Russia 5 A. N. Severtsov Institute of Ecology and Evolution RAS, Moscow, 119071, Russia 6 Conservation Ecology Group, Groningen Institute for Evolutionary Life Sciences (GELIFES), University of Groningen, PO Box 11103, 9700 CC Groningen, The Netherlands *Corresponding author email: jan.van.gils@nioz.nl Manuscript does not contain novel code. Data and script will are publicly available at https://das.nioz.nl (DOI: 10.25850/nioz/7b.b.jd). ______________________________ Ecology_Zhemchuzhnikov_2022.R File contains the script used for data analyses and visualisation. The figures generated with R were afterwards proccessed in Adobe Illustrator. ______________________________ BM_JJ_all.csv File contains the data on phenology of total arthropod biomass, biomass of Tipulidae and biomass of Chironomidae in 2018 and 2019 summer_day - number of the summer day (1st summer day is the 1st of June) year - year of data collection mass - biomass of the arthropods, mg family - family of the arthropod (all - all families, chi - Chironomidae, tip - Tipulidae) peak - biomass during the sampling interval devided by the maximum biomass during the sampling interval (1 - the peak) max - maximum biomass collected during a single sample interval, mg ______________________________ diet_trap_content_chick_growth_period.csv File contains the data on the pitfall traps content and faeces content of the shorebirds' chicks in 2018 and 2019 (table 1 in the MS) order - order of the arthropod family - family of the arthropod (I) Traps, % - relative biomass in the traps (II) Diet, % - relative biomass in the diet based on the number of barcode reads Abbreviations: ReKn - red knot RePh - red phalarope CuSa - curlew sandpiper LiSt - little stint ______________________________ faeces_data.csv File contains the data on faeces content of shorebirds'chicks year - year of data collection brood - brood id chick_no - chick number in the brood sp - shorebird species (RK - red knot, RP - red phalarope, CS - curlew sandpiper, LS - little stint) single_or_pooled - faeces collected from one chick (S) or several chicks (P) year_day - day of the year subsamples_averaged - subsamples of faeces from the same brood were averaged after sequencing (Y - yes, N - no) reads - number of barcode reads family - arthropod family ______________________________ Hatch_food.csv File contains the data on bird and arthropod phenology, and the mismatch year - year sp - bird species (MIN - little stint, PHA - red phalarope, FER - curlew sandpiper, CAN - red knot) hatch - median hatch date (summer day, 1 is the 1st of June) food_sp - peak date of Chironomidae or Tipulidae biomass (summer day, 1 is the 1st of June) family - arthropod family (Chi - Chironomidae, Tip - Tipulidae) total - peak date of total arthropod biomass (summer day, 1 is the 1st of June) mismatch_family - mismatch between median hatch date and peak date of Chironomidae or Tipulidae biomass, days mismatch_total - mismatch between median hatch date and peak date of total arthropod biomass, days ______________________________ insects_data.csv File contains the data on insect samples poskey - id of the sample order - arthropod order family - arthropod family larva - larva (y) or imago (n) length - body length of a random individual of the family in one sample, mm n - number of individuals station.no - pitfall trap number date - date of sampling year - year of sampling status - sampled, placed or covered with snow or water grid.no - number of grid Abbreviations: order Dip - Diptera Col - Coleoptera Hym - Hymenoptera Ara - Araneae Lep - Lepidoptera Ple - Plecoptera Hap - Haplotaxida family Ant - Anthomyiidae Bol - Bolitophilidae Cal - Calliphoridae Car - Carabidae Chi - Chironomidae Chr - Chrysomelidae Dia - Diapriidae Emp - Empididae Ich - Ichneumonidae Lum - Lumbricidae Mus - Muscidae Myc - Mycetophilidae Mym - Mymaridae Nem - Nemouridae Noc - Noctuidae Nym - Nymphalidae Sca - Scathophagidae Sci - Sciaridae Sta - Staphylinidae Syr - Syrphidae Ten - Tenthredinidae Tip - Tipulidae Tri - Trichoceridae unk - unknown ______________________________ length_mass.csv File contains length-mass relationships used for biomass calculation order - arthropod order family - arthropod family b0 - b0 coefficient used for biomass calculation b1 - b1 coefficient used for biomass calculation unit - arthropod taxonomic unit from literature which was used as a reference for calculating of the biomass reference - research article which contains reference for length-weight relationships Abbreviations: Dip - Diptera Col - Coleoptera Hym - Hymenoptera Ara - Araneae Ant - Anthomyiidae Bol - Bolitophilidae Cal - Calliphoridae Car - Carabidae Chi - Chironomidae Chr - Chrysomelidae Dia - Diapriidae Emp - Empididae Ich - Ichneumonidae Mus - Muscidae Myc - Mycetophilidae Mym - Mymaridae Sca - Scathophagidae Sci - Sciaridae Sta - Staphylinidae Syr - Syrphidae Ten - Tenthredinidae Tip - Tipulidae Tri - Trichoceridae unk - unknown ______________________________ phenology_hatch.csv File contains the data on bird phenology Nest - nest id Year - year of data collection Sp - bird species (MIN - little stint, PHA - red phalarope, FER - curlew sandpiper, CAN - red knot) Fate - fate of the nest: (H) hatched, predated (P), deserted (D) or unknown (UnK) HatchDN - hatch day, summer day (1 is the 1st of June)