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  "author": "Daniel  Francois (Royal Netherlands Institute for Sea 
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  "author": "Daniel  Francois (Royal Netherlands Institute for Sea 
            
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Research)",
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Research)",
            
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  "code": "7b.b.xk",
            
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  "contributor": "Royal Netherlands Institute for Sea Research 
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  "contributor": "Royal Netherlands Institute for Sea Research 
            
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(NIOZ)",
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(NIOZ)",
            
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  "creators": "[{\"firstname\":\"Daniel\",\"lastname\":\" 
            
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Francois\",\"orcid\":\"0000-0003-0173-6936\",\"affiliation\":\"Royal 
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Francois\",\"orcid\":\"0000-0003-0173-6936\",\"affiliation\":\"Royal 
            
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Netherlands Institute for Sea 
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Netherlands Institute for Sea 
            
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ddress\":\"daniel.do.nascimento.silva@nioz.nl\",\"formatted\":\"Daniel  
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ddress\":\"daniel.do.nascimento.silva@nioz.nl\",\"formatted\":\"Daniel  
            
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Francois - Royal Netherlands Institute for Sea Research - ORCID: 
13
Francois - Royal Netherlands Institute for Sea Research - ORCID: 
            
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0000-0003-0173-6936 - Corresponding Author - 
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0000-0003-0173-6936 - Corresponding Author - 
            
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daniel.do.nascimento.silva@nioz.nl\"}]",
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daniel.do.nascimento.silva@nioz.nl\"}]",
            
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  "deposit_date": "2026-06-16",
            
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  "depositor": "Daniel do Nascimento Silva 
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  "depositor": "Daniel do Nascimento Silva 
            
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(daniel.do.nascimento.silva@nioz.nl)",
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(daniel.do.nascimento.silva@nioz.nl)",
            
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  "distribution_date": "2026-06-16",
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  "distribution_date": "2026-06-16",
            
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  "distributor": "Research Data Management(NIOZ Royal Netherlands 
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  "distributor": "Research Data Management(NIOZ Royal Netherlands 
            
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Institute for Sea Research)",
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Institute for Sea Research)",
            
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  "doi_date_published": "2026-06-16T10:53:20.0085662",
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  "doi_date_published": "2026-06-16T10:53:20.0085662",
            
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  "maintainer": "Daniel",
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  "maintainer": "Daniel",
            
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  "name": "cryo-fib-sem-foraminifera",
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  "name": "cryo-fib-sem-foraminifera",
            
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  "notes": "Geochemical signatures of the calcium carbonate shells of 
33
  "notes": "Geochemical signatures of the calcium carbonate shells of 
            
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foraminifera form the basis of many proxies used in climate 
34
foraminifera form the basis of many proxies used in climate 
            
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reconstructions. Yet, key physiological controls governing mineral 
35
reconstructions. Yet, key physiological controls governing mineral 
            
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precipitation remain unknown, limiting a mechanistic understanding of 
36
precipitation remain unknown, limiting a mechanistic understanding of 
            
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proxy sensitivity and interspecific variability. Here, we combine 
37
proxy sensitivity and interspecific variability. Here, we combine 
            
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cryo\u2013focused ion beam milling with scanning electron microscopy 
38
cryo\u2013focused ion beam milling with scanning electron microscopy 
            
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to reconstruct the in vivo three-dimensional ultrastructure of the 
39
to reconstruct the in vivo three-dimensional ultrastructure of the 
            
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foraminiferal calcification site. The images reveal a semi-open 
40
foraminiferal calcification site. The images reveal a semi-open 
            
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system, in which the calcifying fluid is largely isolated from ambient 
41
system, in which the calcifying fluid is largely isolated from ambient 
            
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seawater by a thin cellular barrier, while discrete ~30 nm openings 
42
seawater by a thin cellular barrier, while discrete ~30 nm openings 
            
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allow limited fluid exchange. This configuration provides a link 
43
allow limited fluid exchange. This configuration provides a link 
            
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between seawater and the site of calcification, offering a mechanistic 
44
between seawater and the site of calcification, offering a mechanistic 
            
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explanation for the correlation between shell trace-element ratios and 
45
explanation for the correlation between shell trace-element ratios and 
            
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ambient seawater composition, complementing the ion uptake through 
46
ambient seawater composition, complementing the ion uptake through 
            
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transmembrane transport. This, in turn, improves understanding of 
47
transmembrane transport. This, in turn, improves understanding of 
            
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element incorporation and enhances the reliability of foraminiferal 
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element incorporation and enhances the reliability of foraminiferal 
            
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calcite as a paleoproxy.",
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calcite as a paleoproxy.",
            
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