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  "author": "Tim Oortwijn (Royal Netherlands Institute for Sea 
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  "author": "Tim Oortwijn (Royal Netherlands Institute for Sea 
            
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Research)",
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Research)",
            
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  "author_email": "tim.oortwijn@nioz.nl",
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  "author_email": "tim.oortwijn@nioz.nl",
            
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  "code": "7b.b.nk",
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  "code": "7b.b.nk",
            
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  "contributor": "Royal Netherlands Institute for Sea Research 
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  "contributor": "Royal Netherlands Institute for Sea Research 
            
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(NIOZ)",
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(NIOZ)",
            
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  "creators": 
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  "creators": 
            
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\"Oortwijn\",\"orcid\":\"0000-0002-9254-0498\",\"affiliation\":\"Royal 
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\"Oortwijn\",\"orcid\":\"0000-0002-9254-0498\",\"affiliation\":\"Royal 
            
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Netherlands Institute for Sea 
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Netherlands Institute for Sea 
            
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e,\"contactemailaddress\":\"tim.oortwijn@nioz.nl\",\"formatted\":\"Tim 
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e,\"contactemailaddress\":\"tim.oortwijn@nioz.nl\",\"formatted\":\"Tim 
            
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Oortwijn - Royal Netherlands Institute for Sea Research - ORCID: 
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Oortwijn - Royal Netherlands Institute for Sea Research - ORCID: 
            
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0000-0002-9254-0498 - Corresponding Author - 
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0000-0002-9254-0498 - Corresponding Author - 
            
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tim.oortwijn@nioz.nl\"}]",
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tim.oortwijn@nioz.nl\"}]",
            
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  "deposit_date": "2026-02-14",
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  "deposit_date": "2026-02-14",
            
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  "depositor": "Tim Oortwijn (tim.oortwijn@nioz.nl)",
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  "depositor": "Tim Oortwijn (tim.oortwijn@nioz.nl)",
            
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  "distribution_date": "2026-02-14",
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  "distribution_date": "2026-02-14",
            
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  "distributor": "Research Data Management(NIOZ Royal Netherlands 
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  "distributor": "Research Data Management(NIOZ Royal Netherlands 
            
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Institute for Sea Research)",
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Institute for Sea Research)",
            
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  "doi_date_published": "2026-02-14T12:31:12.7806107",
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  "doi_date_published": "2026-02-14T12:31:12.7806107",
            
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  "license_title": null,
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  "maintainer": "Tim",
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  "maintainer": "Tim",
            
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  "metadata_modified": "2026-02-14T11:31:37.247147",
            
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  "name": 
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  "name": 
            
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arly-and-seasonal-variation-in-the-food-of-red-knot-chicks-in-alaska",
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arly-and-seasonal-variation-in-the-food-of-red-knot-chicks-in-alaska",
            
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  "notes": "Studying animal diets using DNA-metabarcoding of faecal 
33
  "notes": "Studying animal diets using DNA-metabarcoding of faecal 
            
34
samples provides a non-invasive, high-resolution approach to identify 
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samples provides a non-invasive, high-resolution approach to identify 
            
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prey. For long-distance migratory shorebirds, such as red knots 
35
prey. For long-distance migratory shorebirds, such as red knots 
            
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(Calidris canutus), chick growth depends on the availability of 
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(Calidris canutus), chick growth depends on the availability of 
            
37
surface-dwelling arthropods during the short Arctic summer, yet the 
37
surface-dwelling arthropods during the short Arctic summer, yet the 
            
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exact diet of the Alaskan-breeding subspecies (C. c. roselaari) 
38
exact diet of the Alaskan-breeding subspecies (C. c. roselaari) 
            
39
remains largely unknown. Using metabarcoding, we characterized the 
39
remains largely unknown. Using metabarcoding, we characterized the 
            
40
diet of red knot chicks in western-Alaska across four summers (2019, 
40
diet of red knot chicks in western-Alaska across four summers (2019, 
            
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2022, 2023 and 2024). We identified key arthropod prey taxa and 
41
2022, 2023 and 2024). We identified key arthropod prey taxa and 
            
42
described how diet composition varied among years and throughout the 
42
described how diet composition varied among years and throughout the 
            
43
chick period. Diet composition was broadly similar between years, but 
43
chick period. Diet composition was broadly similar between years, but 
            
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shifted over the chick-growing period, reflecting temporal changes in 
44
shifted over the chick-growing period, reflecting temporal changes in 
            
45
prey availability. Weevils (Curculionidae) were the most abundant 
45
prey availability. Weevils (Curculionidae) were the most abundant 
            
46
family in the diet (35.8%), especially early in the season, whereas 
46
family in the diet (35.8%), especially early in the season, whereas 
            
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owlet moths (Noctuidae, 11.1%), ichneumoid wasps (Ichneumonidae, 
47
owlet moths (Noctuidae, 11.1%), ichneumoid wasps (Ichneumonidae, 
            
48
8.3%), slugs (Agriolimacidae, 6.5%), house flies (Muscidae, 4.8%), and 
48
8.3%), slugs (Agriolimacidae, 6.5%), house flies (Muscidae, 4.8%), and 
            
49
crane flies (Tipulidae, 4.4%) became more prominent later in the 
49
crane flies (Tipulidae, 4.4%) became more prominent later in the 
            
50
season. These patterns suggest that red knot chicks forage 
50
season. These patterns suggest that red knot chicks forage 
            
51
opportunistically, with diet composition varying over the season in 
51
opportunistically, with diet composition varying over the season in 
            
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response to prey emergence and local environmental conditions. The 
52
response to prey emergence and local environmental conditions. The 
            
53
limited representation of many prey families in pitfall traps 
53
limited representation of many prey families in pitfall traps 
            
54
highlights the need for improved sampling designs to fully capture 
54
highlights the need for improved sampling designs to fully capture 
            
55
prey availability. Together, these findings provide a foundation for 
55
prey availability. Together, these findings provide a foundation for 
            
56
future work on chick growth, survival, and responses to climate-driven 
56
future work on chick growth, survival, and responses to climate-driven 
            
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changes in Arctic ecosystems by tundra-breeding shorebirds.",
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changes in Arctic ecosystems by tundra-breeding shorebirds.",
            
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